diff --git a/backend/src/main/java/com/qqchen/deploy/backend/deploy/repository/ITeamApplicationRepository.java b/backend/src/main/java/com/qqchen/deploy/backend/deploy/repository/ITeamApplicationRepository.java
index efe4c8c2..6f1e194f 100644
--- a/backend/src/main/java/com/qqchen/deploy/backend/deploy/repository/ITeamApplicationRepository.java
+++ b/backend/src/main/java/com/qqchen/deploy/backend/deploy/repository/ITeamApplicationRepository.java
@@ -126,5 +126,15 @@ public interface ITeamApplicationRepository extends IBaseRepository<TeamApplicat
      * @return 团队应用列表
      */
     List<TeamApplication> findByDeploySystemIdAndBuildType(Long deploySystemId, BuildTypeEnum buildType);
+
+    /**
+     * 检查指定团队和环境下是否已存在使用该 Jenkins Job 的配置
+     *
+     * @param teamId 团队ID
+     * @param environmentId 环境ID
+     * @param deployJob Jenkins Job 名称
+     * @return 是否存在
+     */
+    boolean existsByTeamIdAndEnvironmentIdAndDeployJobAndDeletedFalse(Long teamId, Long environmentId, String deployJob);
 }
 
diff --git a/backend/src/main/java/com/qqchen/deploy/backend/deploy/service/impl/TeamApplicationServiceImpl.java b/backend/src/main/java/com/qqchen/deploy/backend/deploy/service/impl/TeamApplicationServiceImpl.java
index 09ea02f6..d05b6abf 100644
--- a/backend/src/main/java/com/qqchen/deploy/backend/deploy/service/impl/TeamApplicationServiceImpl.java
+++ b/backend/src/main/java/com/qqchen/deploy/backend/deploy/service/impl/TeamApplicationServiceImpl.java
@@ -7,6 +7,7 @@ import com.qqchen.deploy.backend.deploy.entity.DeployRecord;
 import com.qqchen.deploy.backend.deploy.entity.Environment;
 import com.qqchen.deploy.backend.deploy.entity.Team;
 import com.qqchen.deploy.backend.deploy.entity.TeamApplication;
+import com.qqchen.deploy.backend.deploy.enums.BuildTypeEnum;
 import com.qqchen.deploy.backend.deploy.query.TeamApplicationQuery;
 import com.qqchen.deploy.backend.deploy.entity.ExternalSystem;
 import com.qqchen.deploy.backend.deploy.entity.RepositoryProject;
@@ -67,12 +68,34 @@ public class TeamApplicationServiceImpl extends BaseServiceImpl<TeamApplication,
     @Override
     @Transactional
     public TeamApplicationDTO create(TeamApplicationDTO dto) {
-        // 检查团队+应用+环境组合是否已存在
-        if (teamApplicationRepository.existsByTeamIdAndApplicationIdAndEnvironmentIdAndDeletedFalse(dto.getTeamId(), dto.getApplicationId(), dto.getEnvironmentId())) {
+        return super.create(dto);
+    }
+
+    /**
+     * 重写父类的唯一性约束校验方法
+     * <p>校验规则：
+     * <ul>
+     *   <li>1. 检查团队+应用+环境组合是否已存在</li>
+     *   <li>2. 检查团队+环境+Jenkins Job 组合是否已存在（仅当构建类型为 JENKINS 时）</li>
+     * </ul>
+     */
+    @Override
+    protected void validateUniqueConstraints(TeamApplicationDTO dto) {
+        // 1. 检查团队+应用+环境组合是否已存在
+        if (teamApplicationRepository.existsByTeamIdAndApplicationIdAndEnvironmentIdAndDeletedFalse(
+                dto.getTeamId(), dto.getApplicationId(), dto.getEnvironmentId())) {
             throw new BusinessException(ResponseCode.TEAM_APPLICATION_ALREADY_EXISTS);
         }
 
-        return super.create(dto);
+        // 2. 检查团队+环境+Jenkins Job 是否已存在（仅当构建类型为 JENKINS 且 deployJob 不为空时）
+        if (dto.getBuildType() == BuildTypeEnum.JENKINS 
+                && dto.getDeployJob() != null 
+                && !dto.getDeployJob().trim().isEmpty()) {
+            if (teamApplicationRepository.existsByTeamIdAndEnvironmentIdAndDeployJobAndDeletedFalse(
+                    dto.getTeamId(), dto.getEnvironmentId(), dto.getDeployJob())) {
+                throw new BusinessException(ResponseCode.TEAM_APPLICATION_DEPLOY_JOB_EXISTS);
+            }
+        }
     }
 
     @Override
diff --git a/backend/src/main/java/com/qqchen/deploy/backend/framework/enums/ResponseCode.java b/backend/src/main/java/com/qqchen/deploy/backend/framework/enums/ResponseCode.java
index d9495ff9..e798a017 100644
--- a/backend/src/main/java/com/qqchen/deploy/backend/framework/enums/ResponseCode.java
+++ b/backend/src/main/java/com/qqchen/deploy/backend/framework/enums/ResponseCode.java
@@ -208,6 +208,7 @@ public enum ResponseCode {
     TEAM_APPLICATION_NOT_FOUND(2927, "team.application.not.found"),
     TEAM_APPLICATION_ALREADY_EXISTS(2928, "team.application.already.exists"),
     TEAM_CONFIG_NOT_FOUND(2929, "team.config.not.found"),
+    TEAM_APPLICATION_DEPLOY_JOB_EXISTS(2930, "team.application.deploy.job.exists"),
 
     // 服务器管理相关错误码 (2950-2969)
     SERVER_NOT_FOUND(2950, "server.not.found"),
diff --git a/backend/src/main/resources/messages.properties b/backend/src/main/resources/messages.properties
index eab4256a..17cd3c4f 100644
--- a/backend/src/main/resources/messages.properties
+++ b/backend/src/main/resources/messages.properties
@@ -275,3 +275,7 @@ jenkins.queue.timeout=Jenkins构建队列超时，等待时间超过{0}秒
 jenkins.build.timeout=Jenkins构建超时：job={0}, buildNumber={1}, 超时时间{2}分钟
 jenkins.api.error=Jenkins API调用失败：HTTP {0}, {1}
 jenkins.response.parse.error=Jenkins响应解析失败：{0}
+
+# 团队应用相关 (2920-2939)
+team.application.already.exists=该应用已配置到此环境
+team.application.deploy.job.exists=该环境下已有其他应用使用此 Jenkins Job，请检查配置
diff --git a/backend/src/main/resources/messages_en_US.properties b/backend/src/main/resources/messages_en_US.properties
index 131a5aa5..3dcffc0c 100644
--- a/backend/src/main/resources/messages_en_US.properties
+++ b/backend/src/main/resources/messages_en_US.properties
@@ -208,3 +208,7 @@ jenkins.queue.timeout=Jenkins build queue timeout, waited more than {0} seconds
 jenkins.build.timeout=Jenkins build timeout: job={0}, buildNumber={1}, timeout {2} minutes
 jenkins.api.error=Jenkins API call failed: HTTP {0}, {1}
 jenkins.response.parse.error=Jenkins response parse error: {0}
+
+# Team Application Related (2920-2939)
+team.application.already.exists=Application already configured for this environment
+team.application.deploy.job.exists=This Jenkins Job is already used by another application in this environment, please check the configuration
diff --git a/backend/src/main/resources/messages_zh_CN.properties b/backend/src/main/resources/messages_zh_CN.properties
index 1897b0c0..4622dd8f 100644
--- a/backend/src/main/resources/messages_zh_CN.properties
+++ b/backend/src/main/resources/messages_zh_CN.properties
@@ -208,3 +208,7 @@ jenkins.queue.timeout=Jenkins构建队列超时，等待时间超过{0}秒
 jenkins.build.timeout=Jenkins构建超时：job={0}, buildNumber={1}, 超时时间{2}分钟
 jenkins.api.error=Jenkins API调用失败：HTTP {0}, {1}
 jenkins.response.parse.error=Jenkins响应解析失败：{0}
+
+# 团队应用相关 (2920-2939)
+team.application.already.exists=该应用已配置到此环境
+team.application.deploy.job.exists=该环境下已有其他应用使用此 Jenkins Job，请检查配置